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10002076

AgraQuant® Crustacea

Allergens Crustacea ELISA Quantitative

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Description & Properties

The AgraQuant® Crustacea is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative analysis of tropomyosin, the major allergen in crustaceans, in food samples. Samples can vary from raw to processed foods, from environmental swabs to rinse water. This product is intended for laboratory use.
Properties
Quantitation range20 - 400 ppb tropomyosin, 0.1 - 2 ppm crustacea protein
Limit of detection0.9 ppb tropomyosin (4.5 ppb crustacea protein)
Limit of quantitation20 ppb (100 ppb crustacea protein)
AllergensCrustacea
Technology/ApplicationELISA
Storage Temperature2°C - 8°C
Result typeQuantitative
Product/EnvironmentEnvironmental Monitoring, Product Testing
Number of wells48

Materials

Materials supplied

  • 48 antibody-coated microwells in a microwell holder sealed in a foil pouch
  • 5 ready-to-use standards (0; 20; 60; 200; and 400 ppb)
  • 1 bottle of concentrated (10X) extraction buffer
  • 1 bottle of concentrated (10X) wash buffer
  • 1 green-capped bottle of conjugate solution
  • 1 blue-capped bottle of substrate solution
  • 1 red-capped bottle of stop solution

Materials required but not supplied

  • Blender
  • analytical balance
  • 8-channel pipette
  • wash bottle
  • microwells/plate reader

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