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10002017

AgraQuant® Histamine

Allergens Histamine ELISA Quantitative

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Description & Properties

The AgraQuant® Histamine is an indirect competitive enzyme-linked immunosorbent assay (ELISA) designed for the quantification of histamine levels in foods, such as fish meal, fresh fish, milk, cheese, sausage and wine. This product is intended for laboratory use.
Properties
Quantitation range0.5 - 50 ppb histamine
Limit of detection0.15 ppb histamine
Limit of quantitation0.5 ppb histamine
AllergensHistamine
Technology/ApplicationELISA
Storage Temperature2°C - 8°C
Result typeQuantitative
Product/EnvironmentEnvironmental Monitoring, Product Testing
Number of wells96

Materials

Materials supplied

  • 96 non-coated reaction plate in a zip-lock bag
  • 96 histamine-coated microwells (12 eight-well strips) in a microwell holder sealed in a foil pouch
  • 6 ready-to-use standards (0; 0.5; 1.5; 5; 15 and 50 ppb)
  • 1 bottle of concentrated (5X) wash buffer
  • 1 bottle of conjugate solution
  • 1 bottle of substrate solution
  • 1 bottle of stop solution
  • 1 bottle of histamine antiserum
  • 2 bottles of acylation buffer
  • 2 bottles of acylation reagent
  • 1 bottle of control 1 (3 ppb ± 1 ppb)
  • 1 bottle of control 2 (10 ppb ± 3 ppb)
  • 1 piece of sealing tape for plate

Materials required but not supplied

  • Blender
  • analytical balance
  • 8-channel pipette
  • wash bottle
  • microwells/plate reader

Documents

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