10001994
AgraQuant® Gluten G12®
Allergens Gluten ELISA Quantitative
Available
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Contact usDescription & Properties
The AgraQuant® Gluten G12® is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative analysis of gluten in a variety of foods. Samples can vary from raw to processed foods, from environmental swabs to rinse water. The G12® antibody targets a 33-mer peptide which is resistant to enzymatic digestion and heat denatiuration, and is the fragment of the gliadin protein to which celiac disease sufferers react, making it a reliable analytical marker. This product is intended for laboratory use.
| Quantitation range | 4 - 200 ppm gluten |
|---|---|
| Limit of detection | 2 ppm gluten |
| Limit of quantitation | 4 ppm gluten |
| Allergens | Gluten |
| Technology/Application | ELISA |
| Storage Temperature | 2°C - 8°C |
| Result type | Quantitative |
| Product/Environment | Environmental Monitoring, Product Testing |
| Approvals | AACC international method 38-52.01, AOAC official method analysis (OMA) 2014.03 |
| Number of wells | 96 |
Materials
Materials supplied
- 96 antibody-coated microwells (12 eight-well strips) in a microwell holder sealed in a foil pouch
- 5 ready-to-use standards (0; 4; 20; 80 and 200 ppm)
- 1 bottle of ready-to-use extraction solution
- 1 bottle of concentrated (5X) diluent buffer
- 1 bottle of concentrated (10X) wash buffer
- 1 green-capped bottle of conjugate solution
- 1 blue-capped bottle of substrate solution
- 1 red-capped bottle of stop solution
- 1 sachet of 10 g fish gelatin
Materials required but not supplied
- Blender
- analytical balance
- 8-channel pipette
- wash bottle
- microwells/plate reader
