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SH-AQ-T2 Toxin

AgraQuant® T-2 Toxin ELISA test

ELISA Quantitative

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Description & Properties

AgraQuant® test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented. These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples.
Properties
Quantitation range20-500 ppb
Limit of detection10 ppb
Limit of quantitation20 ppb
Technology/ApplicationELISA
Storage Temperature2°C - 8°C
Result typeQuantitative
Product/EnvironmentProduct Testing

Materials

Materials supplied in the standard product

  • antibody coated microwells in a microwell holder
  • non-coated dilution microwells
  • 5 vials of each T-2 toxin standard
  • 1 bottle of T-2 toxin conjugate
  • 1 bottle of substrate solution
  • 1 bottle of stop solution

Materials required but not supplied

  • Romer Series II® Mill or equivalent
  • balance - 400 g
  • blender or a tightly sealing jar with lid
  • graduated cylinder: 100mL
  • 70% methanol (ACS grade)
  • distilled or de-ionized water
  • container for filtrate collection with minimum 125mL capacity
  • Whatman#1 filter paper
  • or equivalent
  • filter funnel
  • 8-channel and single channel pipettes capable of pipetting 100µL and 200µL with tips
  • timer
  • wash bottle
  • absorbent paper towels
  • 3 reagent boats for 8-channel pipettes
  • microwell reader with a 450nm filter and an optional differential filter of 630nm or equivalent